Translational_Unit

Part:BBa_K329010

Designed by: Xavier Duportet   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-17)


RBS + Int HK022

This integrase comes from the double-stranded HK022 DNA enterobacteriophage of Escherichia coli, member of the lambdoid or lambda-like group of phages. This protein is necessary for both the integration and the excision of the phage DNA into the host cell. The integration process only requires Int to be expressed, and recombines the attP site from the phage with the attB attachment site on the bacterial chromosome.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None